Table 1 Real-Time PCR Master Mix
Item | Volume |
|---|---|
2× PCR Master Mix (polymerase, dNTPs) | 8 μl |
Sample (cDNA) | 1.6 μl |
Primers (forward/reverse) 1.0 μM (Table 2) | 1.6 μl/1.6 μl |
Distilled water | 3.2 μl |
Item | Volume |
|---|---|
2× PCR Master Mix (polymerase, dNTPs) | 8 μl |
Sample (cDNA) | 1.6 μl |
Primers (forward/reverse) 1.0 μM (Table 2) | 1.6 μl/1.6 μl |
Distilled water | 3.2 μl |