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Table 1 Real-Time PCR Master Mix

From: Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells

Item Volume
2× PCR Master Mix (polymerase, dNTPs) 8 μl
Sample (cDNA) 1.6 μl
Primers (forward/reverse) 1.0 μM (Table 2) 1.6 μl/1.6 μl
Distilled water 3.2 μl