Skip to main content

Table 1 Primers sequences and PCR thermal profile of VDR gene polymorphisms

From: Protective association of VDR gene polymorphisms and haplotypes with multiple sclerosis patients in Egyptian population

SNP Primers sequences and PCR thermal profiles Amplicon (bp)
FokI F 5′-AGC TGG CCC TGG CAC TGA CTC TGC TCT-3′
R 5′-ATG GAA ACA CCT TGC TTC TTC TCC CTC-3′
*Initial denaturation: 95 °C for 10 min; 35 cycles: 94 °C for 30 s, 60 °C for 20 s, and 74 °C for 50 s; and final extension: 74 °C for 5 min
265
BsmI F 5′-GGG AGA CGT AGC AAA AGG-3′
R 5′-AGA GGT CAA GGG TCA CTG-3′
*Initial denaturation 94 °C for 3 min; 32 cycles:94oC for 45 s, 60 °C for 45 s, and 72 °C for 45 s; and final extension: 72 °C for 5 min
358
ApaI and TaqI F 5′-CAG AGC ATG GAC AGG GAG CAA-3′
R 5′-GCA ACT CCT CAT GGC TGA GGT CTC-3′
*Initial denaturation: 94 °C for 10 min; 35 cycles: 94 °C for 15 s, 55 °C for 30 s, and 72 °C for 30 sec; and final extension: 72 °C for 10 min
ApaI = 740
TaqI = 495, 245
Tru9I F 5′-TGA GGT TTC TTG CGG GCA GGG TA-3′
R 5′-CAG GGC CGC CCC TCT TTG GA-3′
*Initial denaturation: 95 °C for 3 min, 35 cycles: 95 °C for 30 s, 72 °C for 60 s, and 72 °C for 60 s; and final extension: 72 °C for 10 mins
212
  1. NCBI official nomenclature, reference sequence AY342401, GI:32891816. SNP single nucleotide polymorphisms, F forward primer sequence, R reverse primer sequence, bp base pair
  2. *: PCR amplification conditions for each SNP