Table 1 Primers sequences and PCR thermal profile of VDR gene polymorphisms

FokI

F 5′-AGC TGG CCC TGG CAC TGA CTC TGC TCT-3′

R 5′-ATG GAA ACA CCT TGC TTC TTC TCC CTC-3′

*Initial denaturation: 95 °C for 10 min; 35 cycles: 94 °C for 30 s, 60 °C for 20 s, and 74 °C for 50 s; and final extension: 74 °C for 5 min

265

BsmI

F 5′-GGG AGA CGT AGC AAA AGG-3′

R 5′-AGA GGT CAA GGG TCA CTG-3′

*Initial denaturation 94 °C for 3 min; 32 cycles:94^oC for 45 s, 60 °C for 45 s, and 72 °C for 45 s; and final extension: 72 °C for 5 min

358

ApaI and TaqI

F 5′-CAG AGC ATG GAC AGG GAG CAA-3′

R 5′-GCA ACT CCT CAT GGC TGA GGT CTC-3′

*Initial denaturation: 94 °C for 10 min; 35 cycles: 94 °C for 15 s, 55 °C for 30 s, and 72 °C for 30 sec; and final extension: 72 °C for 10 min

ApaI = 740

TaqI = 495, 245

Tru9I

F 5′-TGA GGT TTC TTG CGG GCA GGG TA-3′

R 5′-CAG GGC CGC CCC TCT TTG GA-3′

*Initial denaturation: 95 °C for 3 min, 35 cycles: 95 °C for 30 s, 72 °C for 60 s, and 72 °C for 60 s; and final extension: 72 °C for 10 mins

212