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Table 1 Primer sequences for amplification of aminoglycoside resistance genes used in PCR assay

From: Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus species

Target genesPrimer sequences (5′-3′)Amplicon bpReferences
aac(′)-Ie-aph(2′′)-Ia5′-CAGGAATTTATCGAAAATGGTAGAAAAG-3′
5′-CACAATCGACTAAAGAGTACCAATC-3′
369[11]
aph(2′′)-Ib5′-CTTGGACGCTGAGATATATGAGCAC-3′
5′-GTTTGTAGCAATTCAGAAACACCCTT-3′
867
aph(2′′)-Ic5′-CCACAATGATAATGACTCAGTTCCC-3′
5′-CCACAGCTTCCGATAGCAAGAG-3′
444
aph(2′′)-Id5′-GTGGTTTTTACAGGAATGCCATC-3′
5′-CCCTCTTCATACCAATCCATATAACC-3′
641
aph(3′′)-IIIa5′ -GGCTAAAATGAGAATATCACCGG-3′
5′-CTTTAAAAAATCATACAGCTCGCG-3′
523