Table 1 Primer sequences for amplification of aminoglycoside resistance genes used in PCR assay
Target genes | Primer sequences (5′-3′) | Amplicon bp | References |
|---|---|---|---|
aac(′)-Ie-aph(2′′)-Ia | 5′-CAGGAATTTATCGAAAATGGTAGAAAAG-3′ 5′-CACAATCGACTAAAGAGTACCAATC-3′ | 369 | [11] |
aph(2′′)-Ib | 5′-CTTGGACGCTGAGATATATGAGCAC-3′ 5′-GTTTGTAGCAATTCAGAAACACCCTT-3′ | 867 | |
aph(2′′)-Ic | 5′-CCACAATGATAATGACTCAGTTCCC-3′ 5′-CCACAGCTTCCGATAGCAAGAG-3′ | 444 | |
aph(2′′)-Id | 5′-GTGGTTTTTACAGGAATGCCATC-3′ 5′-CCCTCTTCATACCAATCCATATAACC-3′ | 641 | |
aph(3′′)-IIIa | 5′ -GGCTAAAATGAGAATATCACCGG-3′ 5′-CTTTAAAAAATCATACAGCTCGCG-3′ | 523 |