Table 1 Primers sequences for MPL p. W515 L/K pathological variants detection by ARMS technique
From: MPL W515 L/K mutations in myeloproliferative neoplasms
Primer | Sequence |
|---|---|
FO (forward outer) | 5′-GCCTGGATCTCCTTGGTGAC-3′ |
RO (reverse outer) | 5′-GAGGTGACGTGCAGGAAGTG-3′ |
Rwt (reverse wild type) | 5′-CTGTAGTGTGCAGGAAACTGtCA-3′ |
FL (forward mutant L) | 5′-GCCTGCTGCTGCTGAGaTT-3′ |
FK (forward mutant K) | 5′-GCCTGCTGCTGCTGAGtAA-3′ |