Table 2 Primers sequences for JAK2 p.V617F mutation detection by ARMS technique
From: MPL W515 L/K mutations in myeloproliferative neoplasms
Primer | Sequence |
|---|---|
FO (forward outer) | 5′-TCCTCAGAACGTTGATGGCAG-3′ |
RO (reverse outer) | 5′ ATTGCTTTCCTTTTTCACAAGAT-3′ |
Fwt (forward wild type) | 5′-GCATTTGGTTTTAAATTATGGAGTATaTG-3′ |
Rmt (reverse mutant) | 5′ GTTTTACTTACTCTCGTCTCCACAaAA-3′ |