Gene | Length (bp) | Primer’s sequence | Restriction enzyme | Restriction digestion product size (bp) | ||
---|---|---|---|---|---|---|
Normal | Heterozygous | Homozygous | ||||
FVL | 241 | Forward: 5′-TCAGGCAGGAACAAC ACCAT-3′ Reverse: 5′-GGTTACTTCAAGGACA AAATACCTGTAAAGCT-3′ | HindIII | 241 | 241 + 209 + 32 | 209 + 32 |
Prothrombin | 345 | Forward: 5′-TCTAGAAACAGTTGCC TGGC-3′ Reverse: 5′-ATAGCACTGGGAGCAT TGAAGC-3′ | HindIII | 345 | 345 + 322 + 23 | 322 + 23 |