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Table 1 Specific primer sequences, restriction enzymes, and restriction digestion products’ sizes for FVL and prothrombin G20210A. The PCR-RFLP products’ sizes are given according to the genotype polymorphisms

From: Inherited thrombophilia and recurrent pregnancy loss: a single-center case-control study in North-Western Algeria

Gene Length (bp) Primer’s sequence Restriction enzyme Restriction digestion product size (bp)
Normal Heterozygous Homozygous
FVL 241 Forward: 5′-TCAGGCAGGAACAAC
ACCAT-3′
Reverse: 5′-GGTTACTTCAAGGACA
AAATACCTGTAAAGCT-3′
HindIII 241 241 + 209 + 32 209 + 32
Prothrombin 345 Forward: 5′-TCTAGAAACAGTTGCC
TGGC-3′
Reverse: 5′-ATAGCACTGGGAGCAT
TGAAGC-3′
HindIII 345 345 + 322 + 23 322 + 23