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Table 1 Specific primer sequences, restriction enzymes, and restriction digestion products’ sizes for FVL and prothrombin G20210A. The PCR-RFLP products’ sizes are given according to the genotype polymorphisms

From: Inherited thrombophilia and recurrent pregnancy loss: a single-center case-control study in North-Western Algeria

Gene

Length (bp)

Primer’s sequence

Restriction enzyme

Restriction digestion product size (bp)

Normal

Heterozygous

Homozygous

FVL

241

Forward: 5′-TCAGGCAGGAACAAC

ACCAT-3′

Reverse: 5′-GGTTACTTCAAGGACA

AAATACCTGTAAAGCT-3′

HindIII

241

241 + 209 + 32

209 + 32

Prothrombin

345

Forward: 5′-TCTAGAAACAGTTGCC

TGGC-3′

Reverse: 5′-ATAGCACTGGGAGCAT

TGAAGC-3′

HindIII

345

345 + 322 + 23

322 + 23