Table 1 Preparation of standards
Standard | Blood [ml] | Removed plasma (μl) | Phenylalanine stock (μl) | Saline (μl) | Concentration–basal concentration (μmol/l) |
|---|---|---|---|---|---|
A | 20 | 580 | 0 | 580 | 0 |
B | 20 | 580 | 10 | 570 | 50 |
C | 20 | 580 | 20 | 560 | 100 |
D | 20 | 580 | 45 | 535 | 225 |
E | 20 | 580 | 80 | 500 | 400 |
F | 20 | 580 | 160 | 420 | 800 |