Fig. 9From: Effect of Ag nanoparticles on viability of MCF-7 and Vero cell lines and gene expression of apoptotic genesPTEN relative expression in MCF-7 and Vero cell treated or untreated with Ag NPs. Total RNA was extracted, reverse transcribed, and the synthesized DNA was used as a template for qPCR relative expression assay using SYBR green master mix. Data were analyzed by ΔΔ CTs and normalized to (β-actin) house-keeping gene (t test P > 0.05, 0.07)Back to article page