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Egyptian Journal of Medical Human Genetics

Table 2 Thermal cycling of cDNA synthesis (reverse transcriptase)

From: Effect of Ag nanoparticles on viability of MCF-7 and Vero cell lines and gene expression of apoptotic genes

Step

Temperature

Time

Primer annealing ( oligo dT 20)

25

10 min

cDNA synthesis

45

60 min

Heat inactivation

95

5 min

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