Table 4 Components of real-time PCR reaction
Materials | Size |
|---|---|
RealMODTMGreen SF 2X qPCR mix | 10 μl |
Forward for (PTENT, P21, P53, NF-KB, and B-actin) | 1 μl |
Revers for (PTENT, P21, P53, NF-KB, and B-actin) | 1 μl |
Nuclease free water | 7 μl |
Template cDNA | 2 μl |
Final size | 21 μl |