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Egyptian Journal of Medical Human Genetics

Table 5 Real-time PCR program

From: Effect of Ag nanoparticles on viability of MCF-7 and Vero cell lines and gene expression of apoptotic genes

No.

Steps

Temperature

Time

No. of cycles

I

Denaturation 1

94 °C

3 min

1

II

Denaturation

94 °C

30 s

40

III

Annealing

60 °C

30 s

IV

Extension 1

72 °C

1 min

V

Extension 2

72 °C

5 min

1

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