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Egyptian Journal of Medical Human Genetics
Fig. 2 | Egyptian Journal of Medical Human Genetics

Fig. 2

From: The study of sodium and potassium channel gene single-nucleotide variation significance in non-mechanical forms of epilepsy

Fig. 2

PCR-RFLP detection of 26 exon SCN1A gene deletion. a М–GeneRuler Low Range DNA Ladder (Thermo Fisher Scientific, USA); 1—the BamHI restriction results of PCR-amplified products of heterozygote with normal and shorted alleles of SCN1A 26 exon: 140 bp and 108 bp DNA fragments, which are the restriction products of normal allele, and the 215 bp DNA shorted DNA fragment; 2—the 26 exon SCN1A gene PCR-amplified products (248 bp and 215 bp) of patient-carrier of deletion; 3–5—the results of normal PCR-amplified product BamHI restriction: 140 bp and 108 bp DNA fragments; 4–6—the 26 exon SCN1A gene PCR-amplified product (248 bp) of normal sample without mutation; b M–pBR322 DNA/BsuRI (HaeIII) Marker; 1—the 26 exon SCN1A gene PCR-amplified products (248 bp and 215 bp) of patient-carrier of deletion; 2, 4—221 bp DNA fragment used as an additional marker; 3, 5—233 bp DNA fragment used as an additional marker

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