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Egyptian Journal of Medical Human Genetics

Table 1 The site-specific PCR amplification protocols

From: The study of sodium and potassium channel gene single-nucleotide variation significance in non-mechanical forms of epilepsy

Gene, location

SNVs

Primers, 5′→3′

SCN1A, 26 exon

c.5499T>C (p.Phe1820Ser)

f-CCCGACTGTGACCCTAATAAA

r-GTTTGGTTGTGGCAGATTGAG

c.5347G>A (p.Ala1783Thr)

c.5020G>C (p.Gly1674Arg)

f-GTTTCTTGCCGAGCTGATAGA

r-CGATCCCAACTTCCCTCTTAA

c.4969C>Т (p.Pro1657Ala)

f-ACCGGATCCACTGTCTTGATA

r-CGTCTGTAAGCACGCTGAATA

KCNT1, 24 exon

c.2782C>T (p.Arg928Cys)

f-CACCCTGAGACCTCCTACAA

r-CCCTTTCTCCCACTCTTTCTG

c.2800G>A (p.Ala934Thr)

KCNQ2, 5 и 6 exon

c.794 C>T (p.Ala265Val)

f-TGGTGATGCTTCTGGTGATG

r-GTATCAGCAGGGAAAGGGAAA

c.851 A>G (p.Tyr284Cys)

f-GATCACGCTGACCACCATT

r-GGTCCCACCTAGGGAACT

KCNC1, 2 exon

c.959 G>A (p.Arg320His)

f-CTTCGCTTCCCTCTTCTTCAT

r-GAAGATGATGAGCAGCAGGAA

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