Table 2 Restriction endonucleases and SNV specific RFLP details
Gene, location | Mutation/polymorphism//primers (3′→5′) | Restriction endonuclease | DNA fragments length and corresponding genotype |
|---|---|---|---|
SCN1A, 26 exon | c.5459T>C (p.Phe1820Ser) | PstI | TT-321 bp; CC-282 и 39 bp; TC-321, 282 и 39 bp |
c.5347G>A (p.Ala1783Thr) | Acc II | GG-188 и 133 bp; AA-321 bp; GA-321, 188 и 133 bp | |
c.5020G>C (p.Gly1674Arg) | HaeIII | GG-90, 83 и 75 bp; CC -173 и 75 bp; GC-173, 90, 83 и 75 bp | |
c.4969C>Т (p.Pro1657Ala) | BamHI | CC-140 и 108 bp; TT-248 bp; CT-248, 140 и 108 bp. | |
KCNT1, 24 exon | c.2782C>T (p.Arg928Cys) | HpyF10VI | CC-116 и 117 bp; TT-233 bp; CT-233, 117 и 116 bp |
c.2800G>A (p.Ala934Thr) | Acc II | GG-128 и 105 bp; AA-233 bp; GA-233, 128 и 105 bp | |
KCNQ2 5 and 6 exons | c.794 C>T (p.Ala265Val) | RsaI | CC-175 и 35 bp; ТТ-210 bp; СТ-210, 175 и 35 bp |
c.851 A>G (p.Tyr284Cys) | AccII | АА-156 и 65 bp; GG-221 bp; AG-221, 156 и 65 | |
KCNC1 2 exon | c.959 G>A (p.Arg320His) | HpyCH4V | GG-519 bp, AA-367 и 152 bp; GA-519, 367 и 152 |