Long non-coding RNA HOTAIR and HOTTIP as potential biomarkers for hepatitis C virus genotype 4-induced hepatocellular carcinoma

Egyptian Journal of Medical Human Genetics

Table 1 Primers of genes included in the study

Gene	Sequence	Tm	Reference
HOTAIR
Forward	5'-GCA GTA GAA AAA TAG ACA TAG GAGA-3'	58oc	“(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774541/)”
Reverse	5’-AAT GAT AGG GAC ACA TCG GGG AAC T-3’	58oc	“(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774541/)”
HOTTIP
Forward	5’-GTG GGG CCC AGA CCC GC-3’	58oc	“(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637691/)”
Reverse	5’-AAT GAT AGG GAC ACA TCG GGG AAC T-3’	58oc	“(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637691/)”
Β-actin (used as an endogenous control to normalize the amount of total mRNA in each sample)
Forward	5-GCACCACACCTTCTACAATG-3	58oc	“(http://hgsv.washington.edu)”
Reverse	5-TGCTTGCTGATCCACATCTG-3	58oc	“(http://hgsv.washington.edu)”