Fig. 1

STAG3 protein levels in breast cancer cell lines relative to the normal. Protein lysates were extracted from the cell pellets of normal breast and cancer cell lines to study STAG3 protein levels by Western blot. From each cell line, four samples from different flasks were used. a Western blot analysis was performed using Sigma-Aldrich anti-STAG3 antibody to determine the putative protein level of STAG3 in the breast cancer cell lines compared to the positive control, Jurkat cells, and the negative control, MCF-10A. β-Tubulin was used as a loading control. b Western blot analysis was done on two separated protein lysates of each breast cancer cell line and compared to protein lysate extracts of the normal breast and Jurkat cell line. Abcam anti-STAG3 antibody was used to show the putative STAG3 protein level. β-Actin was the loading control. Data shown in a and b represent four experiments for each