Fig. 2
From: Characterization of the major human STAG3 variants using some proteomics and bioinformatics assays
Using Abcam and Sigma anti-STAG3 antibodies for detecting STAG3 protein levels in different cell lines. Following protein lysates extraction from the normal and cancer cell lines, they were run on the same SDS-PAGE gel with the protein size marker loaded in the middle of the gel. Jurkat cells were the positive control and the normal MCF-10A was the negative control. After transfer to a nitrocellulose membrane, it was cut into two pieces from the region of the protein marker. Each part of the membrane was incubated with either Abcam (diluted 1:250) or Sigma (1:2000 dilution) anti-STAG3 antibody