Fig. 4
From: Characterization of the major human STAG3 variants using some proteomics and bioinformatics assays
Western blot analysis for the immunoprecipitated STAG3 protein. Three cancer cell lines, including Jurkat (the positive control), MCF-7, and MDA-MB-231 were used. The STAG3 protein in the lysates (Ly) and those treated with beads and precipitated with either Abcam anti-STAG3 antibody (termed #1) or precipitated with the antibody produced by Sigma (designated #2) as well as beads (B) only was analyzed by Western blot against the antibody manufactured by Sigma to localize protein bands. The upper panel shows blots of the cancer cells exposed to X-ray film for 10 s, while the lower panel refers to the same blot exposed to the film for 30 s. The red arrows represent the postulated STAG3 isoforms