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Egyptian Journal of Medical Human Genetics

Table 1 Features of primer pairs used for the NKX2.5 PCR method

From: Screening of NKX2.5 gene in Moroccan Tetralogy of Fallot (TOF) patients: worldwide mutation rate comparisons show a significant association between R25C variant and TOF phenotype

Exon

Primer sequence (5′ > 3′)

Product size (bp)

1.1*

Fd: CACGA TGCAGGGAAGCTG

477

Rs: AGTTTCTTGGGG ACGAAAGC

1.2**

Fd: CCTCCACGAGGATCCCTTAC

463

Rs: CGAGTCCCCTAGGCATGG

2

Fd: AGAACCGGCGCTACA AGTG

473

Rs: GAGTCAGGGAGCTGTTGAGG

  1. bp base pairs, Fd forward, Rs reverse
  2. *First fragment of exon 1
  3. **Second fragment of exon 1
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