Table 1 Sequences of primers and digestion fragment lengths used for ABCC8 and KCNJ11 genotyping
From: Association of ABCC8 and KCNJ11 gene variants with type 1 diabetes in south Indians
S.No | ABCC8 gene | Primers | Restriction enzyme | Genotype (characterized by fragments in bp) |
|---|---|---|---|---|
1. | -3C > T (rs1799854) | F: 5′-GAG CCA GAG GAG GAT GTT GA3′ R: 5′GGC TAG AAG GAG CGA GGA CT3′ | PstI | CC—248bp CT—248,188 bp TT—188 bp |
2. | C >T (rs1801261) | F-5′TAA AGG CAT GCT CAT GTT GG3′ R-5′AAT GTT CCC AGG ACG CAG TA3′ | BsiE1 | CC—122bp CT—122, 158 bp TT—158 bp |
KCNJ11 gene Allele specific Primers sequences | ||||
1. | G >A (rs5215) | F- TCCAAGTTTGGCAACACCG R - TCCAAGTTTGGCAACACCA Antisense-CTCAGGACAGGGAATCTGGA | Product size: 184 bp | |
2. | C >T (rs5219) | FOP- ATGAGCCACCAGGCCATGGCGAAGAG ROP- AGTGAGGCCCTAGGCCACGTCCGAGG FIP-(C:Allele)- CTGGCGGGCACGGTACCTGGGATC RIP-(T:Allele)- GACACGCCTGGCAGAGGACCCTGACA | Product size: 349 bp C allele: 162 bp T allele: 237 bp | |