Fig. 4From: HLA-A gene knockout using CRISPR/Cas9 system toward overcoming transplantation concernsa Schematic illustration of cleavage positions between exons 2 and 3 (459 bp) and primers locations for detecting desired deletion in HLA-A gene. b Agarose gel electrophoresis of DNA extracted from single-cell colonies are showing colonies with deleted allele (814 bp) and wild-type allele (1273 bp)Back to article page