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Egyptian Journal of Medical Human Genetics
Fig. 1 | Egyptian Journal of Medical Human Genetics

Fig. 1

From: Genetic polymorphism of HLA-G gene (G*01:03, G*01:04, and G*01:05N) in Iraqi patients with inflammatory bowel disease (ulcerative colitis and Crohn’s disease)

Fig. 1

Agarose gel electrophoresis of HLA-G gene PCR products in 3% agarose at 5 V/cm2. a PCR products digested with Hinf-I showing two bands (106 and 175 bp); Lanes 1–14: absence of HLA-G*01:03 allele. b PCR products digested with BseR-I showing three bands (40, 236, and 276 bp bands); lanes 1 and 8: homozygous HLA-G*01:04 allele; lanes 7 and 11: heterozygous HLA-G*01:04 allele; lanes 2–6, 9–10, and 12–13: absence of HLA-G*01:04 allele. c PCR products digested with PpuM-I showing three bands (108, 168, and 276 bp bands); lanes 1-3: homozygous HLA-G*01:05N allele; lanes 7–8 and 10: Heterozygous HLA-G*01:05N allele; lanes 4–6, 9, and 11–13: absence of HLA-G*01:05N allele. Lane M: 100 bp DNA ladder. The bands were visualized using UV trans-illuminator after staining with ethidium bromide

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