Table 2 Assignment of HLA-G*01:03, HLA-G*01:04, and HLA-G*01:05N alleles [25]
Exon | Primer | Restriction endonuclease | Product size (bp) | HLA-G allele |
|---|---|---|---|---|
2 | F: 5′-TCCATGAGGTATTTCAGCGC-3′ | Hinf-I | 79 + 125 | G*01:03 |
R: 5′-CTGGGCCGGAGTTACTCACT-3′ | 106 + 175 | All but G*01:03 | ||
3 | F: 5′-CACACCCTCCAGTGGATGAT-3′ | BseR-I | 276 | G*01:04 |
R: 5′-GGTACCCGCGCGCTGCAGCA-3′ | 40 + 236 | All but G*01:04 | ||
PpuM-I | 276 | G*01:05N | ||
108 + 168 | All but G*01:05N |