Table 1 The primers used in pyrosequencing
From: SCGB3A1 gene DNA methylation status is associated with breast cancer in Egyptian female patients
Gene symbol | Entrez Gene ID | Qiagen product code | Pyrosequencing primers* | Chromosomal location | Number of CpG sites | PCR product size |
|---|---|---|---|---|---|---|
ATM | 4863/472 | Hs_NPAT/ATM_01_PM (PM00153622) | CGCGGACGCGGGAWGGAGGGTTATTGGACCCGGC | Chromosome 11, BP 1080931X-1080932XX | 4 | 203 bp |
SCGB3A1/HIN-1 | 92304 | Hs_SCGB3A1_01_PM (PM0022687) | GGGGCGTTTTTCTGTCTTCGAAACTAGCCTCGACGC | Chromosome 5, BP 1800190XX-1800190XX | 4 | 90 bp |