Prevalence of enterotoxin genes (SEA to SEE) and antibacterial resistant pattern of Staphylococcus aureus isolated from clinical specimens in Assiut city of Egypt

Egyptian Journal of Medical Human Genetics

Table 1 Oligonucleotides primers used in the study

Gene	Primer	Oligonucleotide sequence 5⁻–3⁻	Size of amplified products (bp)	Annealing temp. ^oC	References
SEA	F	GGTTATCAATGTGCGGGTG	102	52	Marsha and Betley [21]
SEA	R	CGGCACTTTTTTCTCTTCGG	102	52	Marsha and Betley [21]
SEB	F	GTATGGTGGTGTAACTGAGC	478	52	Jones and Khan [22]
SEB	R	CCAAATAGTGACGAGTTAGG	478	52	Jones and Khan [22]
SEC	F	AGATGAAGTAGTTGATGTGTATGG	451	50	Bohach and Schlievert [23]
SEC	R	CACACTTTTAGAATCAACCG	451	50	Bohach and Schlievert [23]
SED	F	CCAATAATAGGAGAAAATAAAAG	278	50	Bayles and Iandolo [24]
SED	R	ATTGGTATTTTTTTTCGTTC	278	50	Bayles and Iandolo [24]
SEE	F	AGGTTTTTTCACAGGTCATCC	209	50	Couch et al. [25]
SEE	R	CTTTTTTTTCTTCGGTCAATC	209	50	Couch et al. [25]
16 s rRNA	F	AGAGTTTGATCMTGGCTCAG	1500	55	Turner et al. [27]
16 s rRNA	R	AACTGGAGGAAGGTGGGAT	1500	55	Turner et al. [27]