Egyptian Journal of Medical Human Genetics

Table 1 List of designed, optimized and synthesized primers specific for each gene

From: Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study

Gene name	Forward primer	Reverse primer
IL-1B	GCAATGGTCGGGACATAGTT	AGACCTGACTTGGCAGAGGA
TNF Alpha	ACCACGCTCTTCTGTCTACTG	CTTGGTGGTTTGCTACGAC
PGC-1 Alpha	TTCAGGAGCTGGATGGCTTG	GGGCAGCACACTCTATGTCA
Insulin rats	GTCCTCTGGGAGCCCAAG	ACAGAGCCTCCACCAGG
Glucokinase	GTGTACAAGCTGCACCCGA	CAGCATGCAAGCCTTCTTG
AMPK	ATCCGCAGAGAGATCCAGAA	CGTCGACTCTCCTTTTCGTC
Gsk3b	GGGACAGTGGTGTGGATCAG	GCCGAAAGACCTTCGTCCAA
Glut2	TAGTCAGATTGCTGGCCTCAGCTT	TTGCCCTGACTTCCTCTTCCAAC
PI3K	AACACAGAAGACCAATACTC	TTCGCCATCTACCACTAC
AKT	TCACCTCTGAGACCGACACC	ACTGGCTGAGTAGGAGAACTGG
PTEN	CCCACCACAGCTAGAAACTTATC	CGTCCTTTCCCAGCTTTACA

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