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Table 1 Sequences of designed primers, product sizes and annealing temperatures

From: Molecular study of Pompe disease in Egyptian infants

Fragment

Exon

Primer sequence

Product size (bp)

Annealing (°C)

1

2

F: 5' CTT TGA GAG CCC CGT GAG TG 3'

718

63

R: 5' TTG TGA GGT GCG TGG GTG TC 3'

2

3

F: 5' TCA GAG AGG CTG AAT GTG CTG 3'

306

60

R: 5' GCA CAG AGC CCA GAA CTC AC 3'

3

4 & 5

F: 5' GGT GCG CCT CTT CTG ATA TG 3'

625

60

R: 5' GGA AAT GAG TCC TAT GGG CTG 3'

4

6, 7 & 8

F: 5' TGG GGA GAG AGC CTC AAC TC 3'

653

62

R: 5' GCA CAC ACC ACG ATC ATC ATG 3'

5

9

F: 5' GCT GTA CAC ACG CAT GAT GTC 3'

269

60

R: 5' GCC TCT GCT TTC TAA CCC CC 3'

6

10 & 11

F: 5' TCA GGC TGA GGC TCA GTG GG 3'

465

63

R: 5' CAG AGA TGA GGG TGC TAA GTC 3'

7

12

F: 5' TAA CGC CAG CCC CAC AGA GG 3'

380

60

R: 5' TCC GCT TTT CCT CCT CCC TG 3'

8

13 & 14

F: 5' CAT CAG GTG GCC CAG ACA G 3'

688

62

R: 5' GAG AGT CTT GGG TGG GTG G 3'

9

15

F: 5' ATG CTG GGT GGC TGA GAA G 3'

390

59

R: 5' CCA GGC CCA AAT GTT GTC TC 3'

10

16

F: 5' CCA GCC CCA TCC CAT TCA T 3'

298

59

R: 5' GTT CCT CTT TCC GCC ACC T 3'

11

17

F: 5' AGA TGG AGA GCG TGG TTC C 3'

287

55

R: 5' CCC CAG CTC TGC AGT GTG 3'

12

18 & 19

F: 5' TAG TTA CTG GCA GCC TGG TG 3'

821

57

R: 5' ATC CTC TGT TCC TGG CAT CG 3'

13

20

F: 5' CAC AAT GAC GAC CTC TGA GC 3'

369

56

R: 5' GGA ACC AGC CTC CCT CTG 3'

14*

17, 18 & 19

F: 5' AGA TGG AGA GCG TGG TTC C 3'

Wild-type: 1386

Mutant: 848

56

 

R: 5' ATC CTC TGT TCC TGG CAT CG 3'

  1. *Amplification of this fragment was carried out to investigate the entire deletion of exon 18 (538 bp)