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Table 1 Sequences of designed primers, product sizes and annealing temperatures

From: Molecular study of Pompe disease in Egyptian infants

Fragment Exon Primer sequence Product size (bp) Annealing (°C)
1 2 F: 5' CTT TGA GAG CCC CGT GAG TG 3' 718 63
R: 5' TTG TGA GGT GCG TGG GTG TC 3'
2 3 F: 5' TCA GAG AGG CTG AAT GTG CTG 3' 306 60
R: 5' GCA CAG AGC CCA GAA CTC AC 3'
3 4 & 5 F: 5' GGT GCG CCT CTT CTG ATA TG 3' 625 60
R: 5' GGA AAT GAG TCC TAT GGG CTG 3'
4 6, 7 & 8 F: 5' TGG GGA GAG AGC CTC AAC TC 3' 653 62
R: 5' GCA CAC ACC ACG ATC ATC ATG 3'
5 9 F: 5' GCT GTA CAC ACG CAT GAT GTC 3' 269 60
R: 5' GCC TCT GCT TTC TAA CCC CC 3'
6 10 & 11 F: 5' TCA GGC TGA GGC TCA GTG GG 3' 465 63
R: 5' CAG AGA TGA GGG TGC TAA GTC 3'
7 12 F: 5' TAA CGC CAG CCC CAC AGA GG 3' 380 60
R: 5' TCC GCT TTT CCT CCT CCC TG 3'
8 13 & 14 F: 5' CAT CAG GTG GCC CAG ACA G 3' 688 62
R: 5' GAG AGT CTT GGG TGG GTG G 3'
9 15 F: 5' ATG CTG GGT GGC TGA GAA G 3' 390 59
R: 5' CCA GGC CCA AAT GTT GTC TC 3'
10 16 F: 5' CCA GCC CCA TCC CAT TCA T 3' 298 59
R: 5' GTT CCT CTT TCC GCC ACC T 3'
11 17 F: 5' AGA TGG AGA GCG TGG TTC C 3' 287 55
R: 5' CCC CAG CTC TGC AGT GTG 3'
12 18 & 19 F: 5' TAG TTA CTG GCA GCC TGG TG 3' 821 57
R: 5' ATC CTC TGT TCC TGG CAT CG 3'
13 20 F: 5' CAC AAT GAC GAC CTC TGA GC 3' 369 56
R: 5' GGA ACC AGC CTC CCT CTG 3'
14* 17, 18 & 19 F: 5' AGA TGG AGA GCG TGG TTC C 3' Wild-type: 1386
Mutant: 848
56
  R: 5' ATC CTC TGT TCC TGG CAT CG 3'
  1. *Amplification of this fragment was carried out to investigate the entire deletion of exon 18 (538 bp)