Table 1 Designed primer sequences used in tetra arms PCR genotyping
Gene polymorphism | Primer sequence (5’–3’) | Melting temp (°C) | Amplicons length (bp) |
|---|---|---|---|
eNOS (rs1799983) 894G > T | Forward outer primer | ||
GAGGAGGGCATGAGGCTCAGCCCCAGAA | 63 | ||
Reverse outer primer | 277 | ||
GGATCAGCACCCCCTTGCAGGCCCTTCT | 63 | ||
Forward inner primer (G allele) | |||
CCCCTGCTGCTGCAGGCCCCAGATAAG | 65 | 175 | |
Reverse inner primer (T allele) | |||
CGGGGGGCAGAAGGAAGAGTTCTGGGAGA | 65 | 158 | |
TGFβ1 (rs1800471) 915 C > G | Forward outer primer | ||
CCTCCCCACCACACCAGCCCTGTTCG CG | 62 | ||
Reverse outer primer | 304 | ||
GTACAGGGCGAGCACGGCCTCGGGCAGC | 62 | ||
Forward inner primer (G allele) | |||
GCTGTGGCTACTGGTGCTGACGCCTGGGCG | 66 | 200 | |
Reverse inner primer (C allele) | |||
GCAGGTGGATAGTCCCGCGGCCGCCG | 66 | 160 |