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Egyptian Journal of Medical Human Genetics
Fig. 2 | Egyptian Journal of Medical Human Genetics

Fig. 2

From: A case–control study of BRCA1 founder mutations 185delAG and 5382insC in a cohort of Egyptian ovarian cancer patients using pyrosequencing technique

Fig. 2

Representative pyrogram for BRCA1 185delAG and BRCA1 5382 insC founder mutation. A, B Pyrogram for wild-type sequence for BRCA1 5382 insC. B Pyrogram for heterozygous BRCA1 5382 insC mutation in a carrier FOC patient (case number 1). At position 2 there is an insertion of extra C. Negative internal controls were obtained by negative nucleotide dispensation of G unit at position 1 and T unit at position 3. C At the wild-type sequence for BRCA1 185delAG, the generated peak heights for nucleotide dispensations at positions 2, 3, 4, 5, 6, and 7 (CACTCT in the reverse strand) are approximately equivalent and represent incorporation of a single nucleotide in both alleles (2 light units), but the signal intensity of A at position 9 is two times greater (4 light units) and reflects the incorporation of two as in tandem in both alleles

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