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Egyptian Journal of Medical Human Genetics

Table 2 Primers used in T‐ARMS‐PCR of ghrelin gene (G/T rs696217)

From: Impact of gene polymorphism of glutathione S-transferase and ghrelin as a risk factor in Egyptian women with gestational diabetes mellitus

Primer

Sequence (5′–3′)

Fragment size

FOP

GGGGATTTTTTTTTTTATGGGTTGGTGG

G allele 197 bp (FIP + ROP)

ROP

GGAGGACATTGAGGCAGTAGAGCAGTTG

T allele 266 bp (FOP + RIP)

FIP

GTGGGTTGGTGGTGAGATGTTTACCAATAG

Two outer primer 407

RIP

GTGGACATGAGGGACAAAGTACCCCA

 
  1. FOR forward outer primer, ROP reverse outer primer, FIR forward inner primer, RIP reverse inner primer
  2. aThe mismatches of the allele-specific primers are emphasized in bold and underlined
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