Table 1 Primer sequences, annealing temperatures and amplicon’s bp size used in MSP
From: Significance of promoter methylation of multiple tumor suppressor genes in hepatocellular carcinoma
Genes | Primer | Sequences | Tm (°C) | Size, bp |
|---|---|---|---|---|
RASSF1A | U-F | 5′-TTTGGTTGGAGTGTGTTAATGTG-3′ | 58 |  |
U-R | 5′-CAAACCCCACAAACTAAAAACAA-3′ |  |  | |
M-F | 5′-GTGTTAACGCGTTGCGTATC-3′ | 59 | 203 | |
M-R | 5′-AACCCCGCGAACTAAAAACGA-3′ |  |  | |
CHFR | U-F | 5′-GATTGTAGTTATTTTTGTGATTTGTAGGTGAT-3′ | 56 | 144 |
U-R | 5′-AACTAAAACAAAACCAAAAATAACCCACA-3′ |  |  | |
M-F | 5′-GTTATTTTCGTGATTCGTAGGCGAC-3′ | 58 |  | |
M-R | 5′-CGAAACCGAAAATAACCCGCG-3′ |  |  | |
GSTP1 | U-F | 5’-GATGTTTGGGGTGTAGTGGTTGTT-3′ | 58 | 159 |
U-R | 5′-CCACCCCAATACTAAATCACAACA-3′ |  |  | |
M-F | 5′-TTCGGGGTGTAGCGGTCGTC-3′ | 60 |  | |
M-R | 5′-GCCCCAATACTAAATCACGACG-3′ |  |  | |
MGMT | U-F | 5′-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3′ | 56 | 93 |
U-R | 5′-AACTCCACACTCTTCCAAAAACAAAACA-3′ |  |  | |
M-F | 5′-TTTCGACGTTCGTAGGTTTTCGC-3′ | 59 |  | |
M-R | 5′-GCACTCTTCCGAAAACGAAACG-3′ |  |  | |
hMLH1 | U-F | 5′TTTTGATGTAGATGTTTTATTAGTGGGTTGT-3′ | 56 | 154 |
U-R | 5′-ACCACCTCATCATAACTACCCACA-3′ |  |  | |
M-F | 5′-ACGTAGACGTTTTATTAGGGTCGC-3′ | 62 |  | |
M-R | 5′-CCTCATCGTAACTACCCGCG-3′ |  |  | |
GAPDH NM_002046 | F | 5′TGTAAAACGACGGCCAGT | 58 | 498 |
R | 5′CAGGAAACAGCTATGACC |  |  |