Table 1 The primer sequences and annealing temperatures applied in the PCR reactions
Gene | Primers | Annealing temp (°C) |
|---|---|---|
RUNX3 | ||
Methylated | Sense: 5’-TTACGAGGGGCGGTCGTACGCGGG-3’ Antisense: 5’-AAAACGACCGACGCGAACGCCTCC-3’ | 69.1 |
Unmethylated | Sense: 5’- TTATGAGGGGTGGTTGTATGTGGG-3’ Antisense: 5’- AAAACAACCAACACAAACACCTCC-3’ | 61.8 |
p16 | ||
Methylated | Sense: 5’-TTATTAGAGGGTGGGGCGGATCGC-3’ Antisense: 5’-GACCCCGAACCGCGACCGTAA-3’ | 65 |
Unmethylated | Sense: 5’-TTATTAGAGGGTGGGGTGGATTGT-3’ Antisense: 5’-CAACCCCAAACCACAACCATAA-3’ | 60 |