Skip to main content
Fig. 1 | Egyptian Journal of Medical Human Genetics

Fig. 1

From: B2M gene knockout in HEK293T cells by non-viral delivery of CRISPR-Cas9 system for the generation of universal cells

Fig. 1

A summary of B2M gene knockout as covered in this study indicates the process, which would ultimately lead to gene knockout by CRISPR/Cas9 system. Analysis of the gene deletion via PCR and Sanger sequencing confirms approximately 2.2 bp segment deletion separating the two guide RNAs. Indels in targeted segment is slightly variable between different single clones due the incomplete Cas9 cleavage efficiency

Back to article page