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Egyptian Journal of Medical Human Genetics

Table 1 The guide RNAs targeting the B2M gene

From: B2M gene knockout in HEK293T cells by non-viral delivery of CRISPR-Cas9 system for the generation of universal cells

gRNA name

gRNA sequence

BME

F: 5′-CACCGGGCCGAGATGTCTCGCTCCG- 3'

R: 5′-AAACCGGAGCGAGACATCTCGGCCC- 3'

BMI

F: 5′-CACCGAGCCCCATCAAGAGGTGGAT- 3'

R: 5′-AAACATCCACCTCTTGATGGGGCTC- 3'

  1. BME and BMI indicate the gRNAs targeting exon 1 and intron 1 respectively
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