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Table 2 Primer sequences

From: B2M gene knockout in HEK293T cells by non-viral delivery of CRISPR-Cas9 system for the generation of universal cells

Primer name

Primer sequence

Product length (bp)

CrB2M_1

F: 5′-TTCTTGGGTAGTTTGCAGTTTTAA- 3′

R: 3′-CCCGGCTCTACAGAGCGAGGCCAAA- 5′

141

CrB2M_2

F: 5′-TTCTTGGGTAGTTTGCAGTTTTAA- 3′

R: 3′-CTCGGGGTAGTTCTCCACCTACAAA- 5'

141

CrB2M_3

F: 5′-CCTTGTCCTGATTGGCTGGG-3′

R: 3′-TTTGCGTTTAGGGTGACAGTGTAC-5′

2765

  1. The CrB2M_1 and CrB2M_2 sequences were used to confirm proper integration of the gRNAs into the vector, each targeting exon 1 and intron 1 respectively. The CrB2M_3 served as the primer for genotyping the deleted