Table 2 Primers conditions for quantitative RT-PCR

Primers:

Forward

5′-GAAGCAGGGAGCTTTGAGG -3′

5′-CGCTACAGCCTGAGAGATGA-3′

5′-GGT TTG GCG ATG GCA AGT TC-3′

5′-GACCTGCCGTCTAGAAAAAC-3′

Reverse

5′-ACGATGGGGCAAGTGATG-3′

5′-TCACTCCCTTGGAAGACAGC -3′

5′-TCC CAC CCT AGC TTT CTC CA-3′

5′-TTGAAGTCAGAGGAGACCAC-3′

Thermal profile of qPCR

Initial denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s denaturation, annealing at 60 °C for 10 s and elongation at 72 °C for 10 s