Analysis of missense SNPs in the SLC47A1 and SLC47A2 genes affecting the pharmacokinetics of metformin: Computational approach

Egyptian Journal of Medical Human Genetics

Table 4 Effects of missense SNPs on MATE2 protein stability by MUpro and I-Mutant 3.0

SNP ID	Amino acid change	MUpro result*	Mupro DDG	I-Mutant result^a	I-Mutant DDG	I-Mutant RI
rs111838634	K106E	Decrease	− 0.9204	Decrease	− 0.33	0
rs112362579	I158N	Decrease	− 1.4481	Decrease	− 1.98	5
rs139778919	L112P	Decrease	− 1.786	Decrease	− 1.40	5
rs141717616	P125S	Decrease	− 0.7955	Decrease	− 1.01	7
rs146901447	P162L	Decrease	− 0.1120	Decrease	− 0.80	8
rs147661457	H61Q	Decrease	− 0.4299	Decrease	− 0.34	6
rs148039766	A128E	Decrease	− 0.3744	Decrease	− 0.02	4
rs148775490	G81R	Decrease	− 0.6689	Decrease	− 0.70	4
rs199674976	A298D	Decrease	− 0.6131	Decrease	− 0.64	5
rs373244724	Y273C	Decrease	− 0.7329	Decrease	− 0.91	4
rs374762995	V118G	Decrease	− 2.619	Decrease	− 1.84	10

^aProtein stability: Increase or decrease; DDG values are calculated in kcal/mol