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Table 1 Incubation temperature instructions

From: Intermittent white noise exposure is associated with rat cochleae damage and changes in the gene expression

1. Incubate 10 min at 25 °C

 

2. Incubate 60 min at 47 °C

 

3. Stop the reaction by heating at 85 °C for 5 min

 

4. Chill on ice or at 4 °C