Table 2 The primer sequences of the three genes examined
No. | Gene target | Primer Seq. (5′–3′) | TM | GC% |
|---|---|---|---|---|
1 | Rat GJB2 | F: TCACTGTCCTCTTCATCTTCCG | 60.25 | 50 |
R: CTTCCGTTTCTTTTCGTGTCTC | 58.39 | 45.45 | ||
2 | Rat SLC26A4 | F: CATCATGCCTGGCTGGTTCT | 59.35 | 55 |
R: TGGACACCAACATTCCGTCA | 57.3 | 50 | ||
3 | Rat GAPDH | F: AAGTTCAACGGCACAGTCAAGG | 61.58 | 50 |
R: CATACTCAGCACCAGCATCACC | 61.32 | 54.55 |