Table 1 The PCR primers for real-time PCR (Integrated DNA Technologies, IDT)
From: Generation of muscle progenitors from human-induced pluripotent stem cells
Gene product | Primer forward | Primer reverse | Product size (bp) |
|---|---|---|---|
Myf5 | GAGAGAGCAGGTGGAGAACT | TGGACCAGACAGGACTGTTA | 116 |
Desmin | TACACCTGCGAGATTGACGC | CTGGCAAATCGGTCCTCCAA | 83 |
Dystrophin | TCAGGAGAAAGATGCTGTTTTGC | GGTTCTCAATATGCTGCTTCCC | 182 |
B-Actin | CACAGAGCCTCGCCTTTGC | GAGCGCGGCGATATCATCA | 80 |
PAX7 | TGGCCGGCTATCAGTACG | CAGAGTGCTCCCCGAGCTT | 105 |
MYOD | AAGGCGCCTACTACAACGAG | AGGCAGTCTAGGCTCGACAC | 76 |
MYOGENIN | TCAACCAGGAGGAGCGTGA | TCTGTAGGGTCAGCCGTGAG | 166 |
MYH-2 | CTTTCAAGAGGGACACTGGTCA | TAGCCTGGGAGTGAGACAGTT | 77 |
GAPDH | TTAGGAAAGCCTGCCGGTGA | GGCGCCCAATACGACCAAA | 157 |
Meox1 | CAGGACGAACTCCTCGTCAG | GGCTGAGAAGTCAGGGTACG | 74 |
Meox2 | TGGTTCCAAAACAGGCGGAT | ACCAGTTCCTTTTCCCGAGC | 80 |
PAX3 | CGGCTAGCTGACATTGGTGA | TCCATGTCGTTACTCAGGCG | 96 |
Hand1 | ATATAAGCCAGATCCGCAGGG | TTCTTACCGGTTTCTGCCGC | 90 |
Hand2 | ATTCCGGGTTAGCTTCGGTG | GGAGGCAGAATCCTCTCGTG | 117 |
Mesp1 | CGAGTCCTGGATGCTCTCTG | ATGAGTCTGGGGACGAGACG | 98 |
DKK1 | CGGGCGGGAATAAGTACCAG | GGGACTAGCGCAGTACTCATC | 95 |
Cdh4 | CAAGGATTCGATGAACGGCA | ACACAGCTGCTGAACTTGACT | 87 |
Lhx2 | AACTTCTGTGCCTGGCAACC | AAGAAATTGTGGGGTGAGGGG | 107 |
Myl7 (Mlic-2a) | GGAGTTCAAAGAAGCCTTCAGC | TAGGTCTCCCTCAGGTCTGC | 78 |
Nppa (ANP) | AGCAGTGGATTGCTCCTTGA | GACACGGCATTGTACATGGG | 128 |
Pitx2 | CTCCTGTTCGACCGCCC | AGCCCTGACAGAGAAGTGTC | 151 |
Podxl | AACGAAATCTTGGCCTCGGT | TTGCTGAGCCAGGGTTGTAG | 81 |
Tbx1 | CGAGGAGACACGATTCACCG | TTGCTGGCAATCTTGAGCTG | 72 |
Tcf15 | TGGTCAGAGACAAGGCAGAAC | TCTCCTCAGCTCAGAACGAC | 157 |
Tnnt2 | CATCCAGAAGACAGAGCGGAAAAG | CCTCCTCTCAGCCAGAATCTT | 79 |
Isl1 | TTTCCCTGAATCTCCCCACTC | ACTACACTGAGGCAAACTTCACA | 76 |
NKX2.5 | GTATCCGAGCCTGGTAGGGA | GGACTCAGGGTCATGTTGGG | 112 |
GATA4 | TGGAAGCACCAGGAAGCATT | CAGCCGGTTACAGACGAGAG | 135 |
Mef2c | GCAAAGCCTCGGTCTTCATAGA | ACCAGCTGGCTTTGAAGAGAA | 92 |
Tbx5 | TCAGTCCCCCGGAACAACTC | GGAACTCTTCACGAAGGGAGG | 77 |
Mlc2v (Mylk3) | AGCTTGCACAGATCTTGTCT | CAAACTTAAACCTGGAGGCAGT | 70 |
Myh6 | AGATAGAGAGACTCCTGCGGC | TCTGGGCATCGGTCATCTTG | 87 |
Connexin 43 | TCTGAGTGCCTGAACTTGCC | GGGCACCACTCTTTTGCTTA | 97 |