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Table 1 Primers used in the T-ARMS-PCR for genotyping of FVL G1691A, MTHFRC677T, and FXIIIval34leu gene polymorphisms

From: Association of factor V Leiden R506Q, FXIIIVal34Leu, and MTHFR C677T polymorphisms with acute myocardial infarction

SNP

Primer sequence

Product size (bp)

FVL (rs6025)

Forward inner primer (A allele): GAGCAGATCCCTGGACAGTCA

Common: 242

Reverse inner primer (G allele): ACTTCAAGGACAAAATACCTGTATTCATC

Forward outer primer (5′—3′): GAACATCTTAGAGTTTGATGAACCCAC

G Allele: 175

Reverse outer primer (5′—3′): CCCATTATTTAGCCAGGAGACCTAA

A Allele: 117

MTHFR (rs1801133)

Forward inner primer (T allele): TTGAAGGAGAAGGTGTCTGCGGGCGT

Common: 407

Reverse inner primer (C allele): CAAAGAAAAGCTGCGTGATGATGAAATAGG

Forward outer primer (5′—3′): CCCAGCCACTCACTGTTTTAGTTCAGGC

C Allele: 273

Reverse outer primer (5′—3′): GGTGAGAGTGGGGTGGAGGGAGCTTAT

T Allele: 190

FXIII (rs5985)

Forward inner primer (T allele): CTGCCCACAGTGGAGCTTCAGGACT

Common: 414

Reverse inner primer (G allele): TGACGCCCCGGGGCACTAC

Forward outer primer (5′—3′): CGGCAAAATGTGTTGCTCAAGTGCT

G Allele: 268

Reverse outer primer (5′—3′): TAAAACCAGAGATTGGCAGGGGGCT

T Allele: 190

  1. SNP, single nucleotide polymorphism; bp, base pair; FVL, Factor V Leiden; MTHFR, Methylenetetrahydrofolate reductase