Gene | SNPs | Primers | PCR conditions | RE | Fragment size |
---|---|---|---|---|---|
IL 6 | − 572 | Forward: 5’CTGAAGCAGGTGAAGAAAGT 3’ | 30 cycles: 94ºC 30 s, 52ºC 30 s and 72ºC 30 s | BsrBl | GG:125 bp, 180 bp GC:125 bp, 180 bp,305 bp CC:305 bp |
 | − 597 | Reverse: 5’TTCAGTGACCAGATTAACAGGCTA 3’ |  | Fokl | GG:305 bp, GA:100 bp, 205 bp, 305 bp, AA:100 bp, 205 bp |
IL 8 | − 251 | Forward: 5’CCATCATGATAGCATCTGTA-3’ | 30 cycles: 94ºC 30 s, 54ºC 30 s and 72ºC 30 s | Asel | AA: 21 bp,152 bp, AT: 21 bp,152 bp,173 bp, TT: 173 bp |
 |  | Reverse: 5’-CCACAATTTGGT GAA TTAT*TAA-3’ |  |  |  |
 | 781 | Forward: 5’-CTCTAACTCTTTATATAG GAAT*T-3’ | 30 cycles: 94ºC 30 s, 48ºC 30 s and 72ºC 30 s | EcoRl | TT: 203 bp, TC: 19 bp,184 bp,203 bp, CC: 19 bp,184 bp |
 |  | Reverse: 5’GATTGATTTTATCAACAGGCA-3’ |  |  |  |