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Table 1 Primers and PCR conditions for PCR–RFLP method

From: Association of Interleukin 6 and Interleukin 8 genes polymorphisms with house dust mite-induced nasal-bronchial allergy in a sample of Indian patients

Gene

SNPs

Primers

PCR conditions

RE

Fragment size

IL 6

− 572

Forward: 5’CTGAAGCAGGTGAAGAAAGT 3’

30 cycles: 94ºC 30 s, 52ºC 30 s and 72ºC 30 s

BsrBl

GG:125 bp, 180 bp GC:125 bp, 180 bp,305 bp CC:305 bp

 

− 597

Reverse: 5’TTCAGTGACCAGATTAACAGGCTA 3’

 

Fokl

GG:305 bp, GA:100 bp, 205 bp, 305 bp, AA:100 bp, 205 bp

IL 8

− 251

Forward: 5’CCATCATGATAGCATCTGTA-3’

30 cycles: 94ºC 30 s, 54ºC 30 s and 72ºC 30 s

Asel

AA: 21 bp,152 bp, AT: 21 bp,152 bp,173 bp, TT: 173 bp

  

Reverse: 5’-CCACAATTTGGT GAA TTAT*TAA-3’

   
 

781

Forward: 5’-CTCTAACTCTTTATATAG GAAT*T-3’

30 cycles: 94ºC 30 s, 48ºC 30 s and 72ºC 30 s

EcoRl

TT: 203 bp, TC: 19 bp,184 bp,203 bp, CC: 19 bp,184 bp

  

Reverse: 5’GATTGATTTTATCAACAGGCA-3’

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