Table 1 Oligonucleotide primer sequences, PCR product sizes, and restriction enzymes
SNP | Primer sequence | PCR product size (bp) | RE |
|---|---|---|---|
TLR4 c.896A > G, Asp299Gly(rs4986790) | F:5′- GATTAGCATACTTAGACTACTACCTCCATG-3′ R:5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′ | 249 | NcoI |
TLR4 c.1196 C > T, Thr399Ile (rs4986791) | F:5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA -3′ R:5′-CCTGAAGACTGGAGAGTGAGTTAAATGCT -3′ | 406 | HinfI |