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Table 1 Oligonucleotide primer sequences, PCR product sizes, and restriction enzymes

From: Genetic variation in toll-like receptor 4 gene with primary antiphospholipid syndrome susceptibility: a cohort of Egyptian patients

SNP

Primer sequence

PCR product size (bp)

RE

TLR4 c.896A > G, Asp299Gly(rs4986790)

F:5′- GATTAGCATACTTAGACTACTACCTCCATG-3′

R:5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′

249

NcoI

TLR4 c.1196 C > T, Thr399Ile (rs4986791)

F:5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA -3′

R:5′-CCTGAAGACTGGAGAGTGAGTTAAATGCT -3′

406

HinfI

  1. SNP single nucleotide polymorphism, RE restriction enzyme, TLR4 toll-like receptor 4,
  2. F, forward, R reverse, Underlined bases in both forward primers indicate restriction sites