Table 1 Primer sequences for the selected studied autophagy genes and housekeeping gene
From: Autophagy-related genes in Egyptian patients with Behçet's disease
Gene | Primer |
|---|---|
ATG5 | Forward: 5′-AAAGATGTGCTTCGATGTGT-3′ Reverse: 5 ̀-CACTTTGTCAGTTACCAACGTCA-3′ |
ATG7 | Forward: 5′-ATGATCCCTGTAACTTAGCCCA-3′ Reverse: 5′-CACGGAAGCAAACAAACAACTTCAAC-3′ |
ATG12 | Forward: 5′-TAGAGCGAACACGAACCATCC-3′ Reverse: 5′-CACTGCCAAAACACTCATAGAGA-3′ |
LC3b | Forward: 5′-GATGTCCGACTTATTCGAGAGC-3′ Reverse: 5′-TTGAGCTGTAAGCGCCTTCTA-3′ |
mTOR | Forward: 5′-GCAGATTTGCCAACTTCGG-3′ Reverse: 5′-CAGCGGTAAAAGTGTCCCCTG-3′ |
Actin | Forward: 5′-AGGCCAACCGCGAGAAGATGACC -3′ Reverse: 5′-GAAGTCCAGGGCGACGTAGCAC -3′ |