Explore the distribution of (rs35742686, rs3892097 and rs1065852) genetic polymorphisms of cytochrome P4502D6 gene in the Moroccan population

Egyptian Journal of Medical Human Genetics

Table 1 Primers and restriction enzymes used for genotyping

SNPs CYP2D6	Primers (Tm)	Enzyme	Size (Wild-type)	Size (Heterozygote)	Size (Mutant)
*3 (2549delA)	F/5ʹ-GCTGGGGCCTGAGACTT-3ʹ	BsaAI	201 pb	201-180-20 pb	180-20 pb
*3 (2549delA)	R/5ʹ-GGCTGGGTCCCAGGTCATAC-3ʹ	BsaAI	201 pb	201-180-20 pb	180-20 pb
*4 (G1934A)	F/5ʹ-CCTGGGCAAGAAGTCGCTGGACCAG-3ʹ	BstNI	190-163 pb	353-190-163 pb	353 pb
*4 (G1934A)	R/5ʹ-GAGACTCCTCGGTCTCTCG-3ʹ	BstNI	190-163 pb	353-190-163 pb	353 pb
*10 (C100T)	F/5ʹ-GTGCTGAGAGTGTCCTGCC-3ʹ	HphI	282-62 pb	282-182-100-62 pb	182-100-62 pb
*10 (C100T)	R/5ʹ-CACCCACCATCCATGTTTGC-3ʹ	HphI	282-62 pb	282-182-100-62 pb	182-100-62 pb