Table 1 Primer sequence, restriction enzyme, and cut sequences for each gene

IL-10 ─ 592 C/A

(F: 5´ GGTGAGCACTACCTGACTAAGC 3´)

(R: 5´ CCTAGGTCACAGTGACGTGG 3´)

RsaI

Wild

C

412 bp

Mutant

A

236 bp, 176 bp

IL-10 ─ 819 C/T

(F: 5´ CCAGATATGAAGAAGTCCTG 3´)

(R 5´ TGGGGGAAGTGGGTAAGAGT 3´)

RsaI

Wild

C

443 bp, 116 bp

Mutant

T

559 bp

IL-1ß + 3954 C/T

(F; 5´ TCAGGTGTCCTCGAAGAAATCAAA 3´)

(R: 5´ GCTTTTTTGCTGTGAGTCCCG 3´)

Taq I

Wild

C

97 bp, 85 bp

Mutant

T

182 bp

TNF-α ─ 308 G/A

(F: 5´ ATCCAAGACAACACTACTAA 3´)

(R: 5´ TAAATATCCTCAAAGTTCC 3´)

Nco I

Wild

G

87 bp

Mutant

A

107 bp

TNF-α ─ 857 C/T

(F: 5´ ATCCAAGACAACACTACTAA 3´)

(R: 5´ TAA ATATCCTCAAAGTTCC 3´)

Tai I

Wild

C

18 bp, 109 bp

Mutant

T

127 bp