Table 1 Primers characteristics for methylation-specific PCR
From: Association of methylation status of ABCA1/G1 genes with the risk of coronary artery disease
Gene | Forward primer(5′–3′) | Tm | Reverse primer (5′–3′) | Tm | Product size (bp) |
|---|---|---|---|---|---|
ABCA1 M | 5'TTTGATCGATAGTAATTTTTGC 3' | 51.29 | 5'AAAAATCGACTCGACTCTACG 3' | 55.88 | 126 |
ABCA1 U | 5' GGTTTTGATTGATAGTAATTTTTGT 3' | 53.09 | 5'AAAAAAAATCAACTCAACTCTACA 3' | 53.59 | 126 |
ABCG1 M | 5' ATTTGTATTGTGATATCGACGAGAC 3' | 57.38 | 5'CTTACCTCCTCGATTCTAAACGTAC 3' | 58.80 | 251 |
ABCG1 U | 5'CTTACCTCCTCGATTCTAAACGTAC 3' | 58.80 | 5'AACTTACCTCCTCAATTCTAAACATAC 3' | 57.33 | 251 |