Association of methylation status of ABCA1/G1 genes with the risk of coronary artery disease

Egyptian Journal of Medical Human Genetics

Table 3 Association of ABCA1 gene promoter methylation status with anthropometric and laboratory parameters

Variables	Methylation status		Univariate logistic regression		Multiple logistic regression
Variables	M (80)	U (30)	OR(95%CI)	p	OR(95%CI)*	P*
Age (year)	54.96 ± 8.12	50.23 ± 10.99	1.059 (1.010–1.111)	0.038	0.948 (0.901–0.997)	0.046
Gender (M/F)	50/30	25/5	0.333 (0.115–0.964)	0.037	2.795 (0.914–8.544)	0.071
CAD (yes/no)	56/24	14/16	2.667 (1.126–6.315)	0.023	3.294 (1.233–8.799)	0.017
BMI (kg/m²)	25.61 ± 4.55	24.08 ± 4.32	1.084 (0.980–1.199)	0.115	0.929 (0.832–1.038)	0.193
Systolic blood pressure (mmHg)	134.81 ± 22.10	127.97 ± 20.11	1.016 (0.995–1.037)	0.141	0.990 (0.965–1.015)	0.427
Diastolic blood pressure (mmHg)	81.33 ± 10.05	80.10 ± 12.38	1.011 (0.971–1.052)	0.595
Blood pressure history (yes/no)	36/44	9/21	1.909 (0.779–4.679)	0.154	0.806 (0.295–2.200)	0.674
Smoking (yes/no)	47/33	18/12	0.949 (0.404–2.233)	0.905	1.388 (0.533–3.615)	0.502
Aspirin medication (yes/no)	69/11	23/7	1.909 (0.662–5.503)	0.226
Statin medication (yes/no)	59/21	20/10	1.405 (0.567–3.483)	0.462
TG (mg/dl)	134 (91–180.25)	164 (131.75–192)	2.253 (0.326–15.563)	0.413
Total cholesterol (mg/dl)	154.39 ± 40.34	163.93 ± 36.90	0.994 (0.983–1.005)	0.261
HDL-C(mg/dl)	42.65 ± 10.94	39.22 ± 13.71	1.027 (0.988–1.067)	0.175	0.989 (0.950–1.030)	0.586
LDL-C (mg/dl)	80.85 ± 39.40	97.22 ± 36.80	0.989 (0.979–1.000)	0.051	1.011 (1.000–1.023)	0.058
ApoA1 (mg/dl)	130.65 ± 22.67	130.94 ± 31.22	1.000 (0.983–1.016)	0.958
ApoB (mg/dl)	83.16 ± 27.27	83.80 ± 28.12	0.999 (0.984–1.015)	0.913
ApoB/ApoA1	0.65 ± 0.25	0.68 ± 0.29	0.703 (0.143–3.451)	0.664

M methylated, U unmethylated, CAD coronary artery disease, BMI body mass index, ABCA1 ATP-binding cassette transporters A1, ABCG1 ATP-binding cassette transporters G1, TG triglyceride, HDL-C high-density lipoprotein-cholesterol, LDL-C low-density lipoprotein-cholesterol, ApoA1 apolipoprotein A1, ApoB apolipoprotein B
*OR and P value after adjustment for age, gender, smoking, and aspirin medication
Continuous variables with normal and non-normal distribution are presented as mean ± SD and median (IQR), respectively. Independent Student's t test was performed for normally distributed data and Mann–Whitney U test for non-normally distributed data. Categorical variables are described as number (percentage) using Chi-square test. To determine the relation of ABCA1/G1 methylation and clinical variables, each variable was separately entered in a univariate logistic regression model; then, the variables which had a p < 0.2 were selected and entered into a multiple regression model