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Table 2 Primer sequencing for the Quantitative Methylation Specific PCR (qMSP)

From: Comparative evaluation of ZMYND-8 and RARβ2 genes promoters’ methylation changes in tumor and tumor margin tissues of patients with lung cancer

 

Primer sequence

Annealing temp (°C)

ZMYND-8

Forward:gagtcgtgatggtcgttaagg

Reverse:gtgtcaccacagtaattcgta

59

RAR-B2

Forward:atgttgcgtcgaagtctgaaag

Reverse:ttagtcatcaggattaggatgaag

60