Table 1 Sequences of the tetra primers used in T-ARMS PCR
Primer type | Sequence | Amplicon size (bp) |
|---|---|---|
Forward inner primer (G allele) | 5′-GCTGCTGGTGCAGGGGCCAGGG-3′ | 200 |
Reverse inner primer (C allele) | 5′-CCAGAATGCCAGAGGCTGCTCCGCG-3′ | 247 |
Forward outer primer | 5′-TGCAGGGGGATACGGCCAGGCATTGAAGTC-3′ | 493 |
Reverse outer primer | 5′-TGGGGGGCTGAGGACCTGGTCCTCT-3′ |